Substrate specificity of fatty acid synthetase from yeast
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منابع مشابه
Substrate specificity of fatty acid synthetase from yeast.
The purified multienzyme complex fatty acid synthetase from yeast synthesizes palmitoyland stearoyl-CoA with NADPH, ace@-CoA and malonylCoA as substrates. At least seven different enzymatic steps have been shown to occur in the overall synthesis by the use of appropriate model substrates [l] , .e.g. S-acetoacetyl-N-acetylcysteamine for the @-ketoreductase partial activity. As reported in this c...
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Fatty acid amide hydrolase (FAAH), also referred to as oleamide hydrolase and anandamide amidohydrolase, is a serine hydrolase responsible for the degradation of endogenous oleamide and anandamide, fatty acid amides that function as chemical messengers. FAAH hydrolyzes a range of fatty acid amides, and the present study examines the relative rates of hydrolysis of a variety of natural and unnat...
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Two rat liver fatty acid synthetase preparations, containing 1.6 and 2.0 mol of 4'-phosphopantetheine/mol of synthetase, showed specific activity of 2006 and 2140 nmol of NADPH oxidized/min per mg of protein respectively. The two synthetase preparations could be loaded with either 3.3-4.4 mol of [1-14] acetate or 2.9-3.7 mol of [2-14C]malonate, by incubation with either [1-14C] acetyl-CoA or [2...
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Acetyl coenzyme A synthetase (EC 6.2.1.1) has been examined for its ability to accept various carboxylic acids as substrates in place of acetic acid. The activity of the enzyme with these substrates was monitored using a coupled enzyme assay and high pressure liquid chromatography (HPLC) analysis. Short chain carboxylic acids were found to be active including: propionic, acrylic, fluoroacetic, ...
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It is generally accepted that in the first phase of protein biosynthesis, amino acid-specific enzymes, the aminoacyl-ribonucleic acid synthetases, carry out the activation of the various amino acids, and that the exacting specificity of these enzymes extends to the transfer of the amino acid to a particular soluble ribonucleic acid. Several investigators (l-5) have achieved the purification of ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1972
ISSN: 0014-5793
DOI: 10.1016/0014-5793(72)80696-3